Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Diabetes exacerbates SARS-CoV-2 replication through ineffective pulmonary interferon responses, delayed cell-mediated immunity, and disruption of leptin signaling
doi: 10.3389/fcimb.2025.1513687
Figure Lengend Snippet: Lepr -deficient, T2DM mice show an exacerbated IFN beta pulmonary response following SARS-CoV-2 infection. Relative gene expression of Ifna1 (A) , Ifnb1 (B) , and Ifng (C) in the lungs of mock-infected and SARS-CoV-2-infected lean and Lepr -deficient, T2DM mice at 2 dpi and 4 dpi revealed the activation of Ifnb1 , but not Ifna1 and Ifng . (D) Distribution and (E) abundance of Ifnb1 mRNA in the lung of Lepr -deficient, T2DM, and lean mice by RNAscope ® ISH. Ifnb1 mRNA is primarily expressed in the cytoplasm and nucleus of bronchiolar epithelial cells. × 200 total magnification (Scale bar: 200 μm). Bars represent the mean ± standard deviation. ** P ≤ 0.01; *** P ≤ 0.001.
Article Snippet: The RNAscope ® ISH assay was performed using the RNAscope ® 2.5 LSx reagent kit (Advanced Cell Diagnostics) on the automated BOND RX m platform (Leica Biosystems, Buffalo Grove, IL) as described previously ( ).
Techniques: Infection, Gene Expression, Activation Assay, RNAscope, Standard Deviation